Advanced Fluorescence Microscopy Techniques—FRAP, FLIP, FLAP, FRET and FLIM

Advanced fluorescence microscopy techniques--FRAP, FLIP, FLAP, FRET and FLIM.

Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity. Fluorescence microscopes can both detect the fluorescence emitted from labeled molecules in biological samples as images or photometric data from which intensities and emission spectra can be deduced. By exploiting the characteristics o...

FLAP, FRET and FLIM

Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity. Fluorescence microscopes can both detect the fluorescence emitted from labeled molecules in biological samples as images or photometric data from which intensities and emission spectra can be deduced. By exploiting the characteristics o...

Molecular Imaging: FLIM-FRET Microscopy

Molecular interactions in living cells are dynamic, and techniques that rely on chemical fixation or disruption of cell structure can provide only limited information about these interactions. Technological advances in light microscopy imaging, combined with the availability of genetically encoded fluorescent proteins now provide the tools to obtain spatial and temporal distribution of protein ...

Screening for protein-protein interactions using Förster resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM)

We present a high content multiwell plate cell-based assay approach to quantify protein interactions directly in cells using Förster resonance energy transfer (FRET) read out by automated fluorescence lifetime imaging (FLIM). Automated FLIM is implemented using wide-field time-gated detection, typically requiring only 10 s per field of view (FOV). Averaging over biological, thermal and shot noi...

Corrigendum: Screening for protein-protein interactions using Förster resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM)